Single-Cell Transcriptome Analysis Reveals Six Subpopulations Reflecting Distinct Cellular Fates in Senescent Mouse Embryonic Fibroblasts

transcriptomics

scRNA-seq

senescence

Published

August 11, 2020

Modified

February 11, 2021

Doi

10.3389/fgene.2020.00867

Frontiers in Genetics

Chen W, Wang X, Wei G, Huang Y, Shi Y, Li D, Qiu S, Zhou B, Cao J, Chen M, Qin P, Jin W, Ni T. Single-Cell Transcriptome Analysis Reveals Six Subpopulations Reflecting Distinct Cellular Fates in Senescent Mouse Embryonic Fibroblasts. Front Genet. 2020 Aug 11;11:867. doi: 10.3389/fgene.2020.00867. PMID: 32849838; PMCID: PMC7431633.

They conducted snRNA-seq on Mouse Embryonic Fibroblasts (MEFs) to examine the heterogeneity of single MEFs during cellular senescence. PCA showed that they could be divided into six subpopulations and three distinct lineages during senescence.